Reasearch Awards nomination

Email updates

Keep up to date with the latest news and content from Italian Journal of Pediatrics and BioMed Central.

Open Access Research

Virological and clinical characterizations of respiratory infections in hospitalized children

Suat Bicer1*, Tuba Giray1, Defne Çöl1, Gülay Çiler Erdağ1, Ayça Vitrinel1, Yesim Gürol2, Gülden Çelik2, Çigdem Kaspar3 and Öznur Küçük1

Author Affiliations

1 Yeditepe University, Faculty of Medicine, Department of Child Health and Diseases, Yeditepe University Hospital, Devlet Yolu Ankara Cad. No: 102-104,, Istanbul, Ataşehir, 34752, Turkey

2 Yeditepe University, Faculty of Medicine, Department of Medical Microbiology, Yeditepe University Hospital, Devlet Yolu Ankara Cad. No: 102-104,, Istanbul, Ataşehir, 34752, Turkey

3 Yeditepe University, Faculty of Medicine, Department of Medical Statistics, Yeditepe University Hospital, Devlet Yolu Ankara Cad. No: 102-104, Istanbul, Ataşehir, 34752, Turkey

For all author emails, please log on.

Italian Journal of Pediatrics 2013, 39:22  doi:10.1186/1824-7288-39-22

Published: 27 March 2013

Abstract

Background

The purpose of this study was to determine the incidence and seasonal distribution of viral etiological agents and to compare their clinical manifestations and disease severity, including single and co infections.

Methods

Multiplex reverse-transcription PCR was performed for the detection of viruses in nasopharyngeal aspirat. Disease severity was grouped using a categorization index as very mild/mild, and moderate/severe. Clinical and laboratory characteristics of hospitalized children with viral respiratory tract infection were analyzed.

Results

Viral pathogens were detected in 103/155 (66.5%) of patients. In order of frequency, identified pathogens were respiratory syncytial virus (32.0%), adenovirus (26.2%), parainfluenza viruses type 1–4 (19.4%), rhinovirus (18.4%), influenza A and B (12.6%), human metapneumovirus (12.6%), coronavirus (2.9%), and bocavirus (0.9%). Coinfections were present in 21 samples. Most of the children had very mild (38.8%) and mild disease (37.9%). Severity of illness was not worse with coinfections. The most common discharge diagnoses were "URTI" with or without LRTI/asthma (n=58). Most viruses exhibited strong seasonal patterns. Leukocytosis (22.2%) and neutrophilia (36.6%) were most commonly detected in patients with adenovirus and rhinovirus (p<0.05). Monocytosis was the most remarkable finding in the patients (n=48, 53.3%), especially in patients with adenovirus (p<0.05).

Conclusions

RSV and RhV were associated with higher severity of illness in hospitalized children. RSV found to account for half of LRTI hospitalizations. In AdV and FluA and B infections, fever lasted longer than in other viruses. Coinfections were detected in 21 of the patients. The presence of coinfections was not associated with increased disease severity.

Keywords:
Child; Hospitalization; PCR; Respiratory infections; Viral respiratory pathogens; Respiratory viruses